Batch extraction of β-carotene from Gac ( Momordica cochinchinensis  Spreng) fruit peel

Tran Do Phuong Quyen; Do Hoang Thang; Uyen P.N. Tran; Quoc Hai Nguyen; Hai D. Tran

doi:10.15826/chimtech.2025.12.2.12

Batch extraction of β-carotene from Gac (Momordica cochinchinensis Spreng) fruit peel

Tran Do Phuong Quyen, Do Hoang Thang, Uyen P.N. Tran, Quoc Hai Nguyen, Hai D. Tran

Abstract

β-carotene, a prominent provitamin A carotenoid, functions both as an essential antioxidant beneficial to human health and as a natural food colorant. Gac (Momordica cochinchinensis Spreng) fruit peel, often discarded as biomass waste, represents a promising source of β-carotene. Extracting β-carotene from Gac peel can significantly enhance the value of this waste material. In this study, a conventional solid-liquid method was employed to extract β-carotene from Gac peel. The influence of several factors, including solvent type, particle size, temperature, and solid-to-solvent ratio, on the extraction efficiency was investigated. The results indicated that smaller particle size significantly improved β-carotene extractability. Among acetone, hexane, and ethyl acetate, ethyl acetate proved to be the most effective solvent for β-carotene extraction. At a solvent-to-material ratio of 10:1, the highest β-carotene recovery was attained after three consecutive extractions, with the first extraction accounting for 69.9% of the total recovery. A kinetic model incorporating the effects of driving force and degradation on the kinetics of β-carotene extraction was developed. The calculated activation energies for extraction and degradation were determined to be 9.15 and 69.71 kJ/mol, respectively. This study demonstrates the potential of β-carotene extraction from gac peel and provides valuable insights into the underlying kinetics.

Keywords

Natural product; Carotenoids; Ki-netic model; Biomaterial pro-cessing

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References

Kubola J, Siriamornpun S. Phytochemicals and antioxidant activity of different fruit fractions (peel, pulp, aril and seed) of Thai gac (Momordica cochinchinensis Spreng). Food Chem. 2011;127:1138–1145. doi:10.1016/j.foodchem.2011.01.115

Gul K, Tak A, Singh AK, Singh P, Yousuf B, Wani AA. Chemistry, encapsulation, and health benefits of β-carotene - A review. Cogent Food Agric. 2015;1:1018696.

doi:10.1080/23311932.2015.1018696

Grune T, Lietz G, Palou A, Ross AC, Stahl W, Tang G, Thurnham D, Yin S, Biesalski HK. Beta-carotene is an important vitamin A source for humans. J Nutr. 2010;140:2268S–2285S. doi:10.3945/jn.109.119024

Hoe BC, Arumugam P, Irene Chew ML, Tan J, Ooi CW. Extraction of palm carotene from crude palm oil by solvolytic micellization: economic evaluation and life cycle assessment. Chem Eng Commun. 2022;211(3):336–349. doi:10.1080/00986445.2022.2047664

Chuyen HV, Roach PD, Golding JB, Parks SE, Nguyen MH. Encapsulation of carotenoid-rich oil from Gac peel: Optimisation of the encapsulating process using a spray drier and the storage stability of encapsulated powder. Powder Technol. 2019;344:373–379. doi:10.1016/j.powtec.2018.12.012

Kanda H, Kusumi K, Zhu L, Wang T. Direct extraction of lipids, β-carotene, and polyphenolic compounds from wet microalga Dunaliella salina by liquefied dimethyl ether. Mar Drugs. 2024;22(10):438. doi:10.3390/md22100438

Gungor KK, Torun M. Pumpkin peel valorization using green extraction technology to obtain β-carotene fortified mayonnaise. Waste Biomass Valor. 2022;13:4375–4388. doi:10.1007/s12649-022-01866-y

Jayesree N, Hang PK, Priyangaa A, Krishnamurthy NP, Ramanan RN, Turki MSA, Charis MG, Ooi CW. Valorisation of carrot peel waste by water-induced hydrocolloidal complexation for extraction of carotene and pectin. Chemosphere. 2021;272:129919. doi:10.1016/j.chemosphere.2021.129919

Chifomboti LP, Chimphango AFA. Development and optimization of a two-step co-extraction process for the recovery of pumpkin seed oil and in-situ enrichment with β-carotene compounds from pumpkin peel. Food Bioprod Process. 2025;149:211–223. doi:10.1016/j.fbp.2024.11.015

Hladnik L, Vicente FA, Grilc M, Likozar B. β-Carotene production and extraction: a case study of olive mill wastewater bioremediation by Rhodotorula glutinis with simultaneous carotenoid production. Biomass Conv Bioref. 2024;14:8459–8467. doi:10.1007/s13399-022-03081-0

Hladnik L, Vicente FA, Košir A, Grilc M, Likozar B. Stirred, ultrasound-assisted and microwave-assisted extraction process of β`-carotene from Rhodotorula glutinis in biorefinery downstream. Sep Purif Technol. 2023;311:1232293. doi:10.1016/j.seppur.2023.123293

Ludwig K, Rihko-Struckmann L, Brinitzer G, Unkelbach G, Sundmacher K. β-Carotene extraction from Dunaliella salina by supercritical CO2. J Appl Phycol. 2021:33:1435–1445. doi:10.1007/s10811-021-02399-y

Hagos H, Redi-Abshiro M, Chandravanshi BS, Yaya EE. Development of analytical methods for determination of β-carotene in pumpkin (Cucurbita maxima) flesh, peel, and seed powder samples. Int J Anal Chem. 2022;2022:ID363692. doi:10.1155/2022/9363692

Sun Y, Liu D, Chen J, Ye X, Yu D. Effects of different factors of ultrasound treatment on the extraction yield of the all-trans-β-carotene from citrus peels. Ultrason Sonochem. 2010;18:243–249. doi:10.1016/j.ultsonch.2010.05.014

Hobbi P, Okoro OV, Delporte C, Alimoradi H, Podstawczyk D. Kinetic modelling of the solid–liquid extraction process of polyphenolic compounds from apple pomace: influence of solvent composition and temperature. Bioresour Bioprocess. 2021;8:114. doi:10.1186/s40643-021-00465-4

Natolino A, Porto CD. Kinetic models for conventional and ultrasound assistant extraction of polyphenols from defatted fresh and distilled grape marc and its main components skins and seeds. Chem Eng Res Des. 2020:156:1–12. doi:10.1016/j.cherd.2020.01.009

Das I, Arora A, Kinetics and mechanistic models of solid-liquid extraction of pectin using advance green techniques- a review. Food Hydrocoll. 2021;120:ID106931. doi:10.1016/j.foodhyd.2021.106931

Xu Y, Zhang L, Bailina Y, Ge Z, Ding T, Ye X, Liu D. Effects of ultrasound and/or heating on the extraction of pectin from grapefruit peel. J Food Eng. 2014;126:72–81. doi:10.1016/j.jfoodeng.2013.11.004

Zaid RM, Mishra P, Tabassum S, Wahid ZA, Sakinah AMM. High methoxyl pectin extracts from Hylocereus polyrhizus's peels: Extraction kinetics and thermodynamic studies. Int J Biol Macromol. 2019;141:1147–1157. doi: 10.1016/j.ijbiomac.2019.09.017

Subra P, Castellani S, Jestin P, Aoufi A. Extraction of β-carotene with supercritical fluids: Experiments and modelling. J Supercrit Fluids. 1998;12:261–269. doi:10.1016/S0896-8446(98)00085-0

Döker O, Salgin U, Şanal I, Mehmetoğlu Ü, Çalimli A. Modeling of extraction of β-carotene from apricot bagasse using supercritical CO2 in packed bed extractor. J Supercrit Fluids. 2004;28:11–19. doi:10.1016/S0896-8446(03)00006-8

Takahashi A, Shibasaki‐Kitakawa N, Yonemoto T. Kinetic model for autoxidation of β-carotene in organic solutions. J Am Oil Che Soc. 1999;76:897–903. doi:10.1007/s11746-999-0104-5

Kumar R, Oruna-Concha MJ, Balagiannis DP. Elevated temperature extraction of β-carotene from freeze-dried carrot powder into sunflower oil: Extraction kinetics and thermal stability. J Food Sci. 2024;89(3):1642–1657. doi:10.1111/1750-3841.16964

Tran HD, Nguyen NTT, Phuong TT, Nguyen QH, Dang V-H. Soxhlet extraction of Momordica cochinchinensis fruit peel for β-carotene recovery. RSC Adv. 2025;15(9):6764–6773. doi:10.1039/D4RA08999E

Biswas AK, Sahoo J, Chatli MK. A simple UV-Vis spectrophotometric method for determination of β-carotene content in raw carrot, sweet potato and supplemented chicken meat nuggets. LWT - Food Sci Technol. 2011;44(8):1809–1813. doi:10.1016/j.lwt.2011.03.017

Scott KJ. Detection and Measurement of Carotenoids by UV/VIS Spectrophotometry. Curr Protoc Food Anal Chem. 2001;00:F2.2.1. doi:10.1002/0471142913.faf0202s00

Joda BA, Al-Kadhim ZMA, Ahmed HJ, Al-Khalaf AKH. A convenient green method to synthesize β-carotene from edible carrot and nanoparticle formation. Karbala Int J Mod Sci. 2022;8(3):20–27. doi:10.33640/2405-609X.3200

Tan B, Soderstrom DN. Qualitative aspects of UV-vis spectrophotometry of beta-carotene and lycopene. J Chem Educ. 1989;66:258–260. doi:10.1021/ed066p258

Kopec RE, Cooperstone JL, Cichon MJ, Schwartz SJ. Analysis Methods of Carotenoids. In Analysis of Antioxidant‐Rich Phytochemicals. New York: Wiley;2012, 105–148. doi:10.1002/9781118229378.ch4.

Craft NE, Soares JH. Relative solubility, stability, and absorptivity of lutein and beta-carotene in organic solvents. J Agric Food Chem. 1992;40:431–434. doi:10.1021/jf00015a013

Aris NA, Zaini AS, Nasir HM, Idham Z, Vellasamy Y, Yunus MAC. Effect of particle size and co-extractant in Momordica charantia extract yield and diffusion coefficient using supercritical CO2. Mal J Fund Appl Sci. 2018;14:368–373. doi:10.11113/mjfas.v14n3.1086

Yeop A, Sandanasam J, Pan SF, Abdulla S, Yusoff MM, Gimbun J. The effect of particle size and solvent type on the gallic acid yield obtained from Labisia pumila by ultrasonic extraction. In: Fluids and Chemical Engineering Conference; 2017 April 4-6; Kota Kinabalu, Malaysia. ID02008. doi:10.1051/matecconf/201711102008

Derwenskus F, Metz F, Gille A, Schmid-Staiger U, Briviba K, Schließmann U, Hirth T. Pressurized extraction of unsaturated fatty acids and carotenoids from wet Chlorella vulgaris and Phaeodactylum tricornutum biomass using subcritical liquids. GCB Bioenergy. 2018;11:335–344.

doi: 10.1111/gcbb.12563

Stupar A, Seregelj V, Ribeiro BD, Pezo L, Cvetanović A, Mišan A, Marrucho I. Recovery of β-carotene from pumpkin using switchable natural deep eutectic solvents. Ultrason Sonochem. 2021;76:ID105638. doi:10.1016/j.ultsonch.2021.105638

Wang Y, Mao L, Hu X. Insight into the structural role of carotenoids in the photosystem I: A quantum chemical analysis. Biophys J. 2004;86:3097–3111. doi:10.1016/S0006-3495(04)74358-1

Wang L, Liu Y. Optimization of solvent extraction conditions for total carotenoids in rapeseed using response surface methodology. Na. Sci. 2009;1:23–29. doi:10.4236/ns.2009.11005

Vuong QV, Golding JB, Stathopoulos CE, Nguyen MH, Roach PD. Optimizing conditions for the extraction of catechins from green tea using hot water. J Sep Sci. 2011;34:3099–3106. doi:10.1002/jssc.201000863

Caprio FD, Altimari P, 10.1002/jctb.6464 F. Sequential extraction of lutein and β-carotene from wet microalgal biomass. J Chem Technol Biotechnol. 2020;95(11):3024–3033. doi:10.1002/jctb.6464

Stefanovich AF, Karel M. Kinetics of beta-carotene degradation at temperatures typical of air drying of foods. J Food Process Preserv. 1982;6(4):227–242. doi:10.1111/j.1745-4549.1982.tb00655.x

Wang Y, Wang C, Xue H, Jin Y, Yang M, Leng F. Comparative analysis of three kinds of extraction kinetic models of crude polysaccharides from Codonopsis pilosula and evaluate the characteristics of crude polysaccharides. Biomass Conv Bioref. 2022;13:12917–12933. doi:10.1007/s13399-022-02518-w

Lee WJ, Tan CP, Sulaiman R, Hee YY, Chong GH. Storage stability and degradation kinetics of bioactive compounds in red palm oil microcapsules produced with solution-enhanced dispersion by supercritical carbon dioxide: A comparison with the spray-drying method. Food Chem. 2020;304:ID125427. doi:10.1016/j.foodchem.2019.125427

DOI: https://doi.org/10.15826/chimtech.2025.12.2.12

Copyright (c) 2025 Tran Do Phuong Quyen, Do Hoang Thang, Uyen P.N. Tran, Quoc Hai Nguyen, Hai D. Tran

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